Nutritional 1-C levels through a whole-life feeding trial influence gene expression and DNA methylation patterns in liver and gonad.
Objective
The main goal of NutrEpi WP 1 is to elucidate the effect of micronutrient (vitamins and minerals) on metabolic pathways from a genetic and epigenetic point of view. From previous results from zebrafish studies (Skjærven et al., 2016, Skjærven et al., 2018), it is known that a parental vitamin deficiency affects the embryonic gene expression of immune and lipid related genes.
Experimental design
A whole life cycle feeding trial (over 54 weeks) of Atlantic salmon (Salmo salar) was conducted under the EU funded ARRAINA (advanced research initiatives for nutrition and aquaculture) project (Vera et al., 2020).
Through the trial, triplicate groups - L1, L2, L3 - were fed with graded levels of micronutrient supplements, formulated with nutrition package (NP).
- L1: 100% NP (recommended composition of micronutrients for Atlantic salmon)
- L2: 200% NP
- L3: 400% NP
Project websites
Publication
Micronutrient supplementation affects transcriptional and epigenetic regulation of lipid metabolism in a dose-dependent manner, Takaya Saito, Paul Whatmore, John F Taylor, Jorge M O Fernandes, Anne-Catrin Adam, Douglas R Tocher, Marit Espe and Kaja H Skjærven, Epigenetics (2020),
DOI: 10.1080/15592294.2020.1859867
